Engineered Protein Coatings to Improve the Osseointegration of Dental and Orthopaedic Implants
Identifieur interne : 000855 ( Main/Exploration ); précédent : 000854; suivant : 000856Engineered Protein Coatings to Improve the Osseointegration of Dental and Orthopaedic Implants
Auteurs : Jordan Raphel [États-Unis] ; Johan Karlsson [Suède] ; Silvia Galli [Suède] ; Ann Wennerberg [Suède] ; Christopher Lindsay [États-Unis] ; Matthew Haugh [États-Unis] ; Jukka Pajarinen [États-Unis] ; Stuart B. Goodman [États-Unis] ; Ryo Jimbo [Suède] ; Martin Andersson [Suède] ; Sarah C. Heilshorn [États-Unis]Source :
- Biomaterials [ 0142-9612 ] ; 2016.
Abstract
Here we present the design of an engineered, elastin-like protein (ELP) that is chemically modified to enable stable coatings on the surfaces of titanium-based dental and orthopaedic implants by novel photocrosslinking and solution processing steps. The ELP includes an extended RGD sequence to confer bio-signaling and an elastin-like sequence for mechanical stability. ELP thin films were fabricated on cp-Ti and Ti6Al4V surfaces using scalable spin and dip coating processes with photoactive covalent crosslinking through a carbene insertion mechanism. The coatings withstood procedures mimicking dental screw and hip replacement stem implantations, a key metric for clinical translation. They promoted rapid adhesion of MG63 osteoblast-like cells, with over 80% adhesion after 24 hours, compared to 38% adhesion on uncoated Ti6Al4V. MG63 cells produced significantly more mineralization on ELP coatings compared to uncoated Ti6Al4V. Human bone marrow mesenchymal stem cells (hMSCs) had an earlier increase in alkaline phosphatase activity, indicating more rapid osteogenic differentiation and mineral deposition on adhesive ELP coatings. Rat tibia and femur
Url:
DOI: 10.1016/j.biomaterials.2015.12.030
PubMed: 26790146
PubMed Central: 4771523
Affiliations:
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University, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Materials Science and Engineering, Stanford University, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Karlsson, Johan" sort="Karlsson, Johan" uniqKey="Karlsson J" first="Johan" last="Karlsson">Johan Karlsson</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg</wicri:regionArea><wicri:noRegion>Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Galli, Silvia" sort="Galli, Silvia" uniqKey="Galli S" first="Silvia" last="Galli">Silvia Galli</name><affiliation wicri:level="1"><nlm:aff id="A3">Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö</wicri:regionArea><wicri:noRegion>Malmö</wicri:noRegion></affiliation></author><author><name sortKey="Wennerberg, Ann" sort="Wennerberg, Ann" uniqKey="Wennerberg A" first="Ann" last="Wennerberg">Ann Wennerberg</name><affiliation wicri:level="1"><nlm:aff id="A3">Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö</wicri:regionArea><wicri:noRegion>Malmö</wicri:noRegion></affiliation></author><author><name sortKey="Lindsay, Christopher" sort="Lindsay, Christopher" uniqKey="Lindsay C" first="Christopher" last="Lindsay">Christopher Lindsay</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Materials Science and Engineering, Stanford University, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Haugh, Matthew" sort="Haugh, Matthew" uniqKey="Haugh M" first="Matthew" last="Haugh">Matthew Haugh</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Materials Science and Engineering, Stanford University, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Pajarinen, Jukka" sort="Pajarinen, Jukka" uniqKey="Pajarinen J" first="Jukka" last="Pajarinen">Jukka Pajarinen</name><affiliation wicri:level="2"><nlm:aff id="A4">Department of Orthopaedic Surgery, Stanford University, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Orthopaedic Surgery, Stanford University, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Goodman, Stuart B" sort="Goodman, Stuart B" uniqKey="Goodman S" first="Stuart B." last="Goodman">Stuart B. Goodman</name><affiliation wicri:level="2"><nlm:aff id="A4">Department of Orthopaedic Surgery, Stanford University, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Orthopaedic Surgery, Stanford University, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Jimbo, Ryo" sort="Jimbo, Ryo" uniqKey="Jimbo R" first="Ryo" last="Jimbo">Ryo Jimbo</name><affiliation wicri:level="1"><nlm:aff id="A3">Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Prosthodontics, Faculty of Odontology, Malmö University, Malmö</wicri:regionArea><wicri:noRegion>Malmö</wicri:noRegion></affiliation></author><author><name sortKey="Andersson, Martin" sort="Andersson, Martin" uniqKey="Andersson M" first="Martin" last="Andersson">Martin Andersson</name><affiliation wicri:level="1"><nlm:aff id="A2">Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden</nlm:aff><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg</wicri:regionArea><wicri:noRegion>Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Heilshorn, Sarah C" sort="Heilshorn, Sarah C" uniqKey="Heilshorn S" first="Sarah C." last="Heilshorn">Sarah C. Heilshorn</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Materials Science and Engineering, Stanford University, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Biomaterials</title><idno type="ISSN">0142-9612</idno><idno type="eISSN">1878-5905</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Here we present the design of an engineered, elastin-like protein (ELP) that is chemically modified to enable stable coatings on the surfaces of titanium-based dental and orthopaedic implants by novel photocrosslinking and solution processing steps. The ELP includes an extended RGD sequence to confer bio-signaling and an elastin-like sequence for mechanical stability. ELP thin films were fabricated on cp-Ti and Ti6Al4V surfaces using scalable spin and dip coating processes with photoactive covalent crosslinking through a carbene insertion mechanism. The coatings withstood procedures mimicking dental screw and hip replacement stem implantations, a key metric for clinical translation. They promoted rapid adhesion of MG63 osteoblast-like cells, with over 80% adhesion after 24 hours, compared to 38% adhesion on uncoated Ti6Al4V. MG63 cells produced significantly more mineralization on ELP coatings compared to uncoated Ti6Al4V. Human bone marrow mesenchymal stem cells (hMSCs) had an earlier increase in alkaline phosphatase activity, indicating more rapid osteogenic differentiation and mineral deposition on adhesive ELP coatings. Rat tibia and femur <italic>in vivo</italic> studies demonstrated that cell-adhesive ELP-coated implants increased bone-implant contact area and interfacial strength after one week. These results suggest that ELP coatings withstand surgical implantation and promote rapid osseointegration, enabling earlier implant loading and potentially preventing micromotion that leads to aseptic loosening and premature implant failure.</p></div></front></TEI><affiliations><list><country><li>Suède</li><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Raphel, Jordan" sort="Raphel, Jordan" uniqKey="Raphel J" first="Jordan" last="Raphel">Jordan Raphel</name></region><name sortKey="Goodman, Stuart B" sort="Goodman, Stuart B" uniqKey="Goodman S" first="Stuart B." last="Goodman">Stuart B. Goodman</name><name sortKey="Haugh, Matthew" sort="Haugh, Matthew" uniqKey="Haugh M" first="Matthew" last="Haugh">Matthew Haugh</name><name sortKey="Heilshorn, Sarah C" sort="Heilshorn, Sarah C" uniqKey="Heilshorn S" first="Sarah C." last="Heilshorn">Sarah C. Heilshorn</name><name sortKey="Lindsay, Christopher" sort="Lindsay, Christopher" uniqKey="Lindsay C" first="Christopher" last="Lindsay">Christopher Lindsay</name><name sortKey="Pajarinen, Jukka" sort="Pajarinen, Jukka" uniqKey="Pajarinen J" first="Jukka" last="Pajarinen">Jukka Pajarinen</name></country><country name="Suède"><noRegion><name sortKey="Karlsson, Johan" sort="Karlsson, Johan" uniqKey="Karlsson J" first="Johan" last="Karlsson">Johan Karlsson</name></noRegion><name sortKey="Andersson, Martin" sort="Andersson, Martin" uniqKey="Andersson M" first="Martin" last="Andersson">Martin Andersson</name><name sortKey="Galli, Silvia" sort="Galli, Silvia" uniqKey="Galli S" first="Silvia" last="Galli">Silvia Galli</name><name sortKey="Jimbo, Ryo" sort="Jimbo, Ryo" uniqKey="Jimbo R" first="Ryo" last="Jimbo">Ryo Jimbo</name><name sortKey="Wennerberg, Ann" sort="Wennerberg, Ann" uniqKey="Wennerberg A" first="Ann" last="Wennerberg">Ann Wennerberg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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